BioATRCell
The BioATRCell II allows to follow temperature induced conformational changes of solved and membrane-standing proteins between 0 - 95°C.
The BioATRCell II is a micro-ATR unit that was specially developed for the investigation of temperature induced unfolding, refolding and denaturation processes of proteins in aqueous media. It can be applied for water solved and also for membrane standing proteins. The number of internal reflections in the ATR-crystal (silicon) is adjusted to result in an optimal protein signal in aqueous samples. The BioATRCell II is temperature controlled by the same thermostat like the other accessories that are part of the CONFOCHECK system. With this unit temperature ramps from 0 to 95°C can be performed automatically to analyze the temperature induced structural changes and the protein melting point.
The BioATRCell II can also be used in a flow through manner i.e. for quality control studies of biopharmaceuticals. The required sample volume is about 10 µl. To investigate conformational changes in dependence of pH-changes, salt concentration or ligand binding, a special BioATRCell II with dialysis function is available. This unit allows to dialyze low molecular weight compounds into the protein sample without diluting the protein.
Applications:
- Automated temperature ramps (0-95°C):
Analysis of temperature induced conformational changes
Measurement of phase transitions in lipids - Quality control of biopharmaceuticals
- Membrane standing proteins
- Conformational changes induced by ligand-binding, pH-changes, salts